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goat anti mouse cd32b  (R&D Systems)


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    Structured Review

    R&D Systems goat anti mouse cd32b
    a – d LSECs were isolated using perfusion independent digestion method, followed by positive selection using CD146-magnetic beads. After that, enriched LSECs were placed in culture. a Primary LSEC cultures were stained for an endothelial cell marker (ERG, in green) and nuclear marker (DAPI, in gray). On the left, images show IF-stained LSEC cultures. Scale bar = 25 µm. The yellow arrow highlights an ERG-negative cell. On the right, the dot plot shows the percentage of ERG-positive cells of DAPI-positive cells. [mean ± SD, n = 3 wells]. b Images show IF-stained LSECs with CD31 (in gray), Stabilin-2 (in green), and DAPI (in blue). Scale bar = 25 µm. c Images show IF-stained LSECs with <t>CD32b</t> (in gray) and ERG (in green). Scale bar = 25 µm. d Representative scanning electron microscopy images of cultured LSECs. Scale bar = 2 µm.
    Goat Anti Mouse Cd32b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+cd32b/pmc11711496-253-42-45?v=R%26D+Systems
    Average 93 stars, based on 29 article reviews
    goat anti mouse cd32b - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells"

    Article Title: A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells

    Journal: Communications Biology

    doi: 10.1038/s42003-025-07458-5

    a – d LSECs were isolated using perfusion independent digestion method, followed by positive selection using CD146-magnetic beads. After that, enriched LSECs were placed in culture. a Primary LSEC cultures were stained for an endothelial cell marker (ERG, in green) and nuclear marker (DAPI, in gray). On the left, images show IF-stained LSEC cultures. Scale bar = 25 µm. The yellow arrow highlights an ERG-negative cell. On the right, the dot plot shows the percentage of ERG-positive cells of DAPI-positive cells. [mean ± SD, n = 3 wells]. b Images show IF-stained LSECs with CD31 (in gray), Stabilin-2 (in green), and DAPI (in blue). Scale bar = 25 µm. c Images show IF-stained LSECs with CD32b (in gray) and ERG (in green). Scale bar = 25 µm. d Representative scanning electron microscopy images of cultured LSECs. Scale bar = 2 µm.
    Figure Legend Snippet: a – d LSECs were isolated using perfusion independent digestion method, followed by positive selection using CD146-magnetic beads. After that, enriched LSECs were placed in culture. a Primary LSEC cultures were stained for an endothelial cell marker (ERG, in green) and nuclear marker (DAPI, in gray). On the left, images show IF-stained LSEC cultures. Scale bar = 25 µm. The yellow arrow highlights an ERG-negative cell. On the right, the dot plot shows the percentage of ERG-positive cells of DAPI-positive cells. [mean ± SD, n = 3 wells]. b Images show IF-stained LSECs with CD31 (in gray), Stabilin-2 (in green), and DAPI (in blue). Scale bar = 25 µm. c Images show IF-stained LSECs with CD32b (in gray) and ERG (in green). Scale bar = 25 µm. d Representative scanning electron microscopy images of cultured LSECs. Scale bar = 2 µm.

    Techniques Used: Isolation, Selection, Magnetic Beads, Staining, Marker, Electron Microscopy, Cell Culture



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    Image Search Results


    a – d LSECs were isolated using perfusion independent digestion method, followed by positive selection using CD146-magnetic beads. After that, enriched LSECs were placed in culture. a Primary LSEC cultures were stained for an endothelial cell marker (ERG, in green) and nuclear marker (DAPI, in gray). On the left, images show IF-stained LSEC cultures. Scale bar = 25 µm. The yellow arrow highlights an ERG-negative cell. On the right, the dot plot shows the percentage of ERG-positive cells of DAPI-positive cells. [mean ± SD, n = 3 wells]. b Images show IF-stained LSECs with CD31 (in gray), Stabilin-2 (in green), and DAPI (in blue). Scale bar = 25 µm. c Images show IF-stained LSECs with CD32b (in gray) and ERG (in green). Scale bar = 25 µm. d Representative scanning electron microscopy images of cultured LSECs. Scale bar = 2 µm.

    Journal: Communications Biology

    Article Title: A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells

    doi: 10.1038/s42003-025-07458-5

    Figure Lengend Snippet: a – d LSECs were isolated using perfusion independent digestion method, followed by positive selection using CD146-magnetic beads. After that, enriched LSECs were placed in culture. a Primary LSEC cultures were stained for an endothelial cell marker (ERG, in green) and nuclear marker (DAPI, in gray). On the left, images show IF-stained LSEC cultures. Scale bar = 25 µm. The yellow arrow highlights an ERG-negative cell. On the right, the dot plot shows the percentage of ERG-positive cells of DAPI-positive cells. [mean ± SD, n = 3 wells]. b Images show IF-stained LSECs with CD31 (in gray), Stabilin-2 (in green), and DAPI (in blue). Scale bar = 25 µm. c Images show IF-stained LSECs with CD32b (in gray) and ERG (in green). Scale bar = 25 µm. d Representative scanning electron microscopy images of cultured LSECs. Scale bar = 2 µm.

    Article Snippet: Cells were subsequently washed with PBS, fixed in 4% PFA [Carl Roth, Cat #0335.1], blocked with 10% normal donkey serum [Biozol, Cat #LIN-END9010-10] and incubated in a primary antibody mix consisting of different combinations of goat anti-mouse CD31 [R&D Systems, Cat #AF3628], goat anti-mouse CD32b [R&D Systems, Cat #AF1460], rabbit anti-mouse Desmin [Abcam, Cat # ab15200], rabbit anti-mouse ERG [Abcam, Cat #ab196149], rat anti-mouse F4/80 [BioLeegend, # 123102], and rabbit anti-mouse Stabilin-2 antibodies overnight.

    Techniques: Isolation, Selection, Magnetic Beads, Staining, Marker, Electron Microscopy, Cell Culture